Coding

Part:BBa_K2429042:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-24)


pTRE L. shahii dCas13a-DDX6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 6173
    Illegal XbaI site found at 30
    Illegal PstI site found at 2205
    Illegal PstI site found at 2574
    Illegal PstI site found at 3303
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 6173
    Illegal PstI site found at 2205
    Illegal PstI site found at 2574
    Illegal PstI site found at 3303
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 6173
    Illegal BglII site found at 949
    Illegal BglII site found at 1573
    Illegal BglII site found at 1969
    Illegal BglII site found at 2260
    Illegal BglII site found at 2350
    Illegal BglII site found at 3511
    Illegal BglII site found at 3598
    Illegal BamHI site found at 339
    Illegal BamHI site found at 4593
    Illegal BamHI site found at 4695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 6173
    Illegal XbaI site found at 30
    Illegal PstI site found at 2205
    Illegal PstI site found at 2574
    Illegal PstI site found at 3303
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 6173
    Illegal XbaI site found at 30
    Illegal PstI site found at 2205
    Illegal PstI site found at 2574
    Illegal PstI site found at 3303
    Illegal NgoMIV site found at 4550
    Illegal NgoMIV site found at 4569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 935
    Illegal SapI.rc site found at 1041
    Illegal SapI.rc site found at 1911
    Illegal SapI.rc site found at 2745
    Illegal SapI.rc site found at 6051


Design Notes

Human Codon Optimized


Source

The catalytically active sequence of the protein came from L. shahii Cas13a on Addgene(https://www.addgene.org/79150/), which is also where our team received the original plasmid from. DDX6 seqeuence was taken from another plasmid received from another researcher in lab


References